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I wanted to be her. I wanted to drive a convertible Lexus. I wanted to be the most popular girl in school. And if I needed to be a bimbo to get there, I’d oblige. Scheme 1 (A) Schematic illustration of the synthesis of HA +, fabrication of the UnTHCPSi nanoparticles from boron doped p+ Si <100> wafers, conjugation of HA + onto the surface of the UnTHCPSi nanoparticles via EDC/NHS coupling chemistry. (B) Hypothesis of the application of the resulting UnTHCPSi–HA + nanoparticles for the targeting of CD44-overexpressing breast cancer cells.
Q. Xu, M. Hashimoto, T. T. Dang, T. Hoare, D. S. Kohane, G. M. Whitesides, R. Langer and D. G. Anderson, Small, 2009, 5, 1575–1581 CrossRef CAS PubMed. Fig. 1 (A) TEM images of the UnTHCPSi and UnTHCPSi–HA + nanoparticles. Scale bars are 100 nm. (B) Colloidal stability of UnTHCPSi and UnTHCPSi–HA + after incubation in HEPES buffer (pH 7.4) at room temperature for 4 h. (C) FTIR absorbance spectra of HA (a), HA + (b), UnTHCPSi–HA + (c), and UnTHCPSi (d). The relevant features of each spectrum are highlighted and numbered for clarity (see text for details). J. Salonen, A. M. Kaukonen, J. Hirvonen and V.-P. Lehto, J. Pharm. Sci., 2008, 97, 632–653 CrossRef CAS PubMed.A buy-in management buyout (BIMBO) occurs when an outside management team joins a company (buying-in) while also buying out the existing management team.
K. Hettiarachchi, S. Zhang, S. Feingold, A. P. Lee and P. A. Dayton, Biotechnol. Prog., 2009, 25, 938–945 CrossRef CAS PubMed.Bimbo is slang for a conventionally attractive, sexualized, naive, and unintelligent woman. [1] The term was originally used in the United States as early as 1919 for an unintelligent or brutish man. [2] L. M. Bimbo, E. Mäkilä, T. Laaksonen, V.-P. Lehto, J. Salonen, J. Hirvonen and H. A. Santos, Biomaterials, 2011, 32, 2625–2633 CrossRef CAS PubMed. In American politics, the word was used in the 1990s during Bill Clinton's sexual misconduct allegations, leading to the invention of the term " Bimbo eruptions" to refer to political sex scandals. [14] The expression was also used in a 2014 report [15] in which Colin Powell explained his reluctance to vote for Hillary Clinton in light of her husband's continued affairs with "bimbos". Players were responsible for feeding their bimbo, maintaining her mood, and keeping her clean and healthy. They could earn Bimbo Attitude (BA) and IQ points by playing mini-games or spending time at locations in the city. For instance, bimbos could earn IQ by spending an hour in the library or earn BA by paying for plastic surgery at the clinic or changing their style at the salon. Bimbos could also accumulate BA regularly from their boyfriends and homes.
Justin Cord, The Unexpected Evolution of Language: Discover the Surprising Etymology of Everyday Words Hayes Adams Media, Sep 18, 2012 Fig. 3 In vitro cytotoxicity of UnTHCPSi and UnTHCPSi–HA + nanoparticles, after incubation with MDA-MB-231 and MCF-7 breast cancer cell lines for 6 and 24 h at 37 °C. The cell viability was evaluated by an ATP-based luminescent assay after exposing the cells to different concentrations of the nanoparticles. Cells cultured with HBSS (pH 7.4) and with 1% Triton X-100 were used as positive and negative controls, respectively. Error bars represent mean ± s.d. ( n ≥ 3). Statistical analysis was performed by means of the Student's t-test with the level of significance set at probabilities of * p< 0.05, ** p< 0.01, and *** p< 0.001. The size and morphology of the UnTHCPSi and UnTHCPSi–HA + nanoparticles were analyzed by transmission electron microscopy (TEM). The TEM pictures were captured using a Jeol JEM-1400 microscope (Jeol Ltd., Tokyo, Japan) at an 80 kV voltage. The nanoparticle suspensions were centrifuged, re-dispersed in ethanol at a concentration of 10 μg mL −1, and dropped on a carbon-coated copper TEM grid, followed by drying for 48 h at room temperature. Stability studies in human plasma For performing these experiments, 300 μg of UnTHCPSi and UnTHCPSi–HA + were dispersed in 200 μL of 1× PBS (pH 7.4), exposed to 1500 μL of human plasma, and left under stirring at 800 rpm and 37 °C for 2 h. Sample aliquots of 200 μL were withdrawn at pre-determined time points (1, 5, 10, 15, 30, 60, 90, and 120 min), and the corresponding size, ζ-potential, and PdI were subsequently determined by DLS and ELS. The results are shown as the average of at least three independent measurements. Anonymous human plasma donors were obtained from the Finnish Red Cross Blood Service, with the permission from the respective institutional ethical committee. Cell lines and culturing conditions For the in vitro studies, MCF-7 and MDA-MB-231 breast cancer cells were cultured according to the protocols described in detail in the ESI. † In vitro cytotoxicity studies The in vitro cytotoxicity of both UnTHCPSi and UnTHCPSi–HA + was evaluated by a CellTiter-Glo ® Luminescent Cell Viability assay, as previously described elsewhere. 26,29 MCF-7 and MDA-MB-231 breast cancer cells were suspended in the corresponding cell culture media at a concentration of 2 × 10 5 cells per mL, and approximately 2 × 10 4 cells per well were seeded in 96-well plates (Corning Inc. Life Sciences, USA). The cells were allowed to attach overnight at 37 °C, after which the cell culture medium was removed and replaced with 100 μL of UnTHCPSi and UnTHCPSi–HA + nanoparticle suspensions at concentrations of 25, 50, and 100 μg mL −1, with 1× HBSS (pH 7.4) and 1% Triton X-100 as positive and negative controls, respectively. After incubating for 6 and 24 h at 37 °C, 100 μL of the assay reagent was added to each well and the number of viable cells was determined by measuring the luminescence from the living cells using a Varioskan Flash fluorometer (Thermo Fisher Scientific Inc., USA). The results presented correspond to the average of at least three independent measurements. Cellular internalization The cellular uptake and intracellular localization of the UnTHCPSi and UnTHCPSi–HA + nanoparticles was assessed by TEM. Approximately 10 5 cells per well of MCF-7 and MDA-MB-231 breast cancer cells were seeded in 24-well plates (Corning Inc. Life Sciences, USA) with each well containing a 13 mm round shaped coverslip and allowed to attach overnight at 37 °C. After removing the cell culture media, 500 μL per well of the nanoparticle suspensions at a concentration of 50 μg mL −1 were added to the wells. After an incubation period of 6 h at 37 °C, the nanoparticle suspensions were carefully removed and the samples were rinsed twice with HBSS–HEPES (pH 7.4). Then, the cells were fixed with 2.5% glutaraldehyde in 0.1 M PBS buffer (pH 7.4) for 1 h at room temperature, and subsequently washed twice with HBSS–HEPES (pH 7.4) and sodium cacodylate buffer (NaCac) for 3 min, prior to post-fixation with 1% osmium tetroxide in 0.1 M NaCac buffer (pH 7.4). Thereafter, the cells were dehydrated with 30–100% ethanol for 10 min and embedded in epoxy resin. Ultrathin sections with approximately 60 nm were sliced parallel to the coverslips, post-stained with uranyl acetate and lead citrate, and finally analyzed by TEM as described above. Flow cytometric analysis of CD44 expression and cellular association The expression of CD44 receptor in MCF-7 and MDA-MB-231 breast cancer cell lines was evaluated by flow cytometry according to a protocol adapted from the manufacturer's instructions, as described in the ESI. † L. M. Bimbo, M. Sarparanta, E. Mäkilä, T. Laaksonen, P. Laaksonen, J. Salonen, M. B. Linder, J. Hirvonen, A. J. Airaksinen and H. A. Santos, Nanoscale, 2012, 4, 3184–3192 RSC. Lemon fic containing the carnal 'hobbies' of princesses, princes and villains. Erotic fiction. I own nothing. No Flames! Just tell me who you want to see and what you want them to be doing.Miss Bimbo started as an English version of a French game called Ma Bimbo. [1] Ma Bimbo was owned and developed by Beemoov and Miss Bimbo was developed by Blouzar Ltd., London. Around the same time, another drama erupted, though less intense: the travel agency drama. Although this travel agency has been teased since 2008, mentions of it were systematically banned as well as the members bringing it up. One player famously got banned for saying that the travel agency won't open til 2010... Turns out this player was right since the travel agency finally opened in late 2010. They were many artists in the art section of the forum, some which were professionals or currently training to be, new terms and conditions actually made it that each and every piece of art no matter what, even if watermarked, was owned and reusable as they wish by the developers. You can imagine how THAT turned out (it pretty much wiped the entire art community, because even those who stayed couldn't really find the joy with the drama and most people who were active who left) AB - Substitution of fossil fuels by sustainable practices must be rapidly implemented to mitigate the impacts of climate change. The conversion of biomass into combustible gas is investigated in a microwave-induced plasma reactor using pure steam as the plasma working gas for the first time. The optimum results are achieved at the highest forward microwave power of 6 kW with biomass carbon conversion efficiency over 98% and complete biomass energy recovery in syngas. Unreacted steam is simply condensed out, leading to the production of a syngas with low inert dilution and high calorific value in the range 10.5–12 MJ/Nm3. The syngas produced is rich in hydrogen, exceeding 60% by volume. The proposed process could aid in the transition to a carbon neutral economy as it has the potential to efficiently convert biomass to syngas that can be used for the sustainable generation of fuels, chemicals and energy. Fletcher reminds us that it’s important to keep the historical context in mind too: “The new age movement can’t be totally unproblematic. It stands to reason that not every feminist movement will resonate with every kind of woman.”
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